PPIP html report

Introduction

This report is designed to facilitate summarization and rapid browsing of complex results in PPIP pipelines.

1 QC

Quality control (QC) are necessary to facilitate successful genomics and proteomics analyses. This procedure will check the produced data to decide whether these are reliable enough to be used, and help to improve the arguments of tools in the Pipeline.

1.1 Quality control of NGS data

Quality control and filtering of sequencing reads is one of the most important steps in the pre-processing of sequencing reads. In this sub-section, the AfterQC (https://github.com/OpenGene/AfterQC), a tool with functions to profile sequencing errors and correct most of them, plus highly automated quality control and data filtering features will be used.

Click me to view the QC report

• 131012_I266_FCC2J5JACXX_L6_RSCOaowTAARAAPEI-72_1.fq.gz.html

1.2 Peptide-level qvalue distribution

This plot depicts the Q-value distribution of all PSMs (peptide spectrum match). In this pipeline, we used the Q-value <= 0.01 to find the truly significant results. The blue area is the density distribution of all Q-values. And the green curve represents the related empirical cumulative distribution of all Q-value.

1.3 Precursor error distribution

This graph displays a precursor mass error distribution in MS-level. As shown in the figure, the blue curve represents the distribution of all the unfiltered PSMs. And the red curve indicates the trace of PSMs satisfied with statistical criteria.

2 Basic result

The main purpose of this chapter is to describe the basic statistical analysis of endogenous peptides.

2.1 Identified peptide length distribution

Amino acid length distribution of the identified peptides.

2.2 Spectral count of identified peptides

Spectral count, defined as the total number of spectra identified for a peptide, has gained acceptance as a practical in proteomic studies. As a supplement to Q-value, the more count a peptide has, the more credible the peptide is.

2.3 Amino acid distribution of C-/N-term

To view the amino acid number distribution of N-terminus and C-terminus, we depict a barplot as follows:

2.4 Cysteine distribution of peptides

During the maturation process of proteins in eukaryote, cysteine residues are oxidised and form disulfide bridges, which are important to maintain protein shapes. Through this shape, disulfide bonds play en essential role for the stability and the activity of proteins. The following graph use an pie to describe how many cysteine residues in each peptide.

2.5 Summary table of peptides

This table includes the peptide identification result that could be used for further analysis.

Peptide	Protein	PepQValue	SpecCount
CGPCFTTDANMAR	NTX312243	0.0	38
SDDSFTDDLNKRPDQRFSF	NTX496322	0.0	34
LFKLATKIIPSLFR	NTX134676	0.0	20
NDDIEEPFIKKNAYVTG	NTX384939	0.0	20
CGPCFTTDANMARK	NTX312243	0.0	19
NDDIEEPFIKKNAY	NTX384939	0.0	18
LDEDDEAILKKLAIEELKNE	NTX574605	0.0	15
NDDIEEPFIKKNA	NTX384939	0.0	14
LISKEEFIKMT	NTX56407	0.0	13
NDDIEEPFIKKN	NTX384939	0.0	11
SDDSFTDDLNKRPDQRF	NTX496322	0.0	11
IDLPDKVPIRIP	NTX602038	0.0	10
MGYAPYDYREEGHLT	NTX112170	0.0	10
MSEHIFKETDKNNDY	NTX56407	0.0	10
TPFEVRCATDAD	NTX416183	0.0	10
ETKGGNLDWDQLIGEKL	NTX487647	0.0	9
GDCPSLNEPCDSDRDCCPYGERCLSSGKGYYCKEDPGP	NTX366384	0.0	9
IDLPDKVPIRIPGPC	NTX602038	0.0	9
TDDLNKRPDQRFSF	NTX496322	0.0	9
CGPCFTTDANMARKC	NTX312243	0.0	8
FDPAIFGSLPDDTKE	NTX581382	0.0	7
FFGHLFKLAT	NTX134676	0.0	7
SMDGDMEDYMKRRFSF	NTX163969	0.0	7
DLFYHVCRCY	NTX359850	0.0	6
FDSVSAAGAGRF	NTX479826	0.0	6
GDCPSLNEPCDSDRDCCPYGE	NTX366384	0.0	6
NGVDKKYDDYGHM	NTX416169	0.0	6
SDSVYVIDKIIRP	NTX135011	0.0	6
SDSVYVIDKIIRPVLKDDLKE	NTX135011	0.0	6
TDEELAEYEKMIAQ	NTX56407	0.0	6
VEDFPGKLDEDDEAILKKLAIEELKNE	NTX574605	0.0	6
AILTPEVNKVYE	NTX56407	0.0	5
CLSSGKGYYCKEDPGP	NTX366384	0.0	5
DDIEEPFIKKNAY	NTX384939	0.0	5
KIVDGIKSYNIE	NTX91175	0.0	5
SQFKDEFVFCFIQSHA	NTX660903	0.0	5
VIWAEQQVVKPRV	NTX487637	0.0	5
YCRPDFGP	NTX627879	0.0	5
CISTGVNYYCRPDFGP	NTX627879	0.0	4
DIEEPFIKKNAY	NTX384939	0.0	4
DLNKRPDQRFSF	NTX496322	0.0	4
FSFGLGKRPDQRFAF	NTX496322	0.0	4
FYHVCRCY	NTX359850	0.0	4
GLGKRPDQRFAF	NTX496322	0.0	4
KGHYTEGAELVDSVLDVVR	NTX324164	0.0	4
LDEDDEAILKKLAIEE	NTX574605	0.0	4
LFKLATKIIPSLF	NTX134676	0.0	4
LVKYAVPEGTLR	NTX444153	0.0	4
LWGNEGCNKECRAYGAS	NTX41957	0.0	4
MGYAPYDYREEGH	NTX112170	0.0	4
NANGVDKKYDDYGHM	NTX416169	0.0	4
RKMKDTDSEEEIREAF	NTX248037	0.0	4
SDDSFTDDLNKRPDQRFSFGLG	NTX496322	0.0	4
SDSVYVIDKIIRPVLKDDL	NTX135011	0.0	4
VEAILTPEVNKVYE	NTX56407	0.0	4
VIWAEQQVVKPR	NTX487637	0.0	4
WIPSDYGGHM	NTX14137	0.0	4
WIPSDYGGHMN	NTX14137	0.0	4
DSFTDDLNKRPDQRFSF	NTX496322	0.0	3
DSVYVIDKIIRP	NTX135011	0.0	3
EFAGKKISEIEIVNLEDAHEGVV	NTX612360	0.0	3
FDPTSEAGAGRFDS	NTX479826	0.0	3
FQLPSHLDIRNPHTFEEEDL	NTX56407	0.0	3
GDCPSLNEPCDSDRD	NTX366384	0.0	3
GLGKRPDQRFSF	NTX496322	0.0	3
LECPTLYQR	NTX592223	0.0	3
LFENNHVLPK	NTX385699	0.0	3
LITDRHVLTAAHC	NTX142744	0.0	3
LNEQETYAADITPPHPQKHPYWY	NTX28979	0.0	3
LNNPDRKAFQLPSHLDI	NTX56407	0.0	3
LSPAVLASLTGLEKQ	NTX581382	0.0	3
LVKYAVPEGTLRT	NTX444153	0.0	3
LVKYAVPEGTLRTIIQ	NTX444153	0.0	3
LWGNEGCNKECRA	NTX41957	0.0	3
SDDSFTDDLNKRPDQRFSFGLGKRPDQ	NTX496322	0.0	3
SDSVYVIDKIIRPVLKDD	NTX135011	0.0	3
SMFDDGYPVKNGCRIS	NTX249700	0.0	3
TDEELAEYEKMIAQK	NTX56407	0.0	3
YYCRPDFGP	NTX627879	0.0	3
AFGLGKRPDQRFSF	NTX496322	0.0	2
ATGGNRTKTPGPGAQSALRALAR	NTX519666	0.0	2
CIKYSWTCKFN	NTX154016	0.0	2
DEFNPETFFSMHD	NTX56407	0.0	2
DEVEAILTPEVNKVYE	NTX56407	0.0	2
DGYIRGSNGCKVSCL	NTX596227	0.0	2
EDEVEAILTPEVNKVYE	NTX56407	0.0	2
EEDTCNLFMCSMYCRFKE	NTX637329	0.0	2
EVEAILTPEVNKVYE	NTX56407	0.0	2
FAFGLGKRPDQRFSF	NTX496322	0.0	2
FDPTLEAGAGRFNPALEAGAGRFDPASEAGAGR	NTX591828	0.0	2
FIILVGTKWCGAGN	NTX644817	0.0	2
FIQDNTYEIYDKSKQ	NTX181117	0.0	2
FQLPSHLDIRNP	NTX56407	0.0	2
GGYYGYCYFWK	NTX434966	0.0	2
HDDNGHFTNDWVVR	NTX487637	0.0	2
IIGFQLGLERPTNVVQNNTS	NTX624266	0.0	2
KISEIEIVNLEDAHEGVV	NTX612360	0.0	2
LGKRPDQRFAF	NTX496322	0.0	2
LLGCDHDGEKGSLDCSARDGY	NTX177486	0.0	2
LSSGKGYYCKEDPGP	NTX366384	0.0	2
LWGNEGCNKECRAY	NTX41957	0.0	2
LWGNEGCNKECRAYGA	NTX41957	0.0	2
SDDSFTDDLNKRPDQ	NTX496322	0.0	2
SDDSFTDDLNKRPDQRFS	NTX496322	0.0	2
SDNPSPVEDIEGEGKVDD	NTX583217	0.0	2
SFGLGKRPDQRF	NTX496322	0.0	2
SMRDMDTMKY	NTX372159	0.0	2
SRNDDIEEPFIKKNAY	NTX384939	0.0	2
TDEELAEYEKMI	NTX56407	0.0	2
TQEKEQIKTLNNRFA	NTX577282	0.0	2
TQTDGCGPCFTTDANMAR	NTX312243	0.0	2
VWEEEDELPK	NTX56407	0.0	2
YAPYDYREEGH	NTX112170	0.0	2
YEQNLAEKFERPQIT	NTX352134	0.0	2
AFIGAVAGLLSK	NTX372159	0.0	1
AFQLPSHLDIRNPHTFEEEDL	NTX56407	0.0	1
DCCPYGERCLSSGKGYYCKEDPGP	NTX366384	0.0	1
DDLNKRPDQRFSF	NTX496322	0.0	1
EHGIITNWDDMEKIWHHT	NTX24145	0.0	1
FDPTSEAGAGRFDSVSAAGAGR	NTX479826	0.0	1
FQLPSHLDIRNPHTFE	NTX56407	0.0	1
GDCPSLNEPCDSDRDCCPYGERCLS	NTX366384	0.0	1
GDCPSLNEPCDSDRDCCPYGERCLSSGKGYYCKED	NTX366384	0.0	1
GICVVETENDLFYHVCRCY	NTX359850	0.0	1
ISTGVNYYCRPDFGP	NTX627879	0.0	1
IVIYNMEGSGPNTTPKLKAGI	NTX407408	0.0	1
KAFQLPSHLDIRNPHTFEEEDL	NTX56407	0.0	1
KAKLEETHPERVEAF	NTX431293	0.0	1
KEDKYEEEIKVLTDK	NTX186740	0.0	1
KIVDGIKSYNIEREEEL	NTX91175	0.0	1
LASELGYYNLGELKGFKDTY	NTX487637	0.0	1
LAVPHDVYPGYGITK	NTX13418	0.0	1
LEPAGDVISDKF	NTX135011	0.0	1
LNNPDRKAFQLPSHLD	NTX56407	0.0	1
LNPYCNDLCTKN	NTX83905	0.0	1
LSISPPRLYPR	NTX597930	0.0	1
LTDKESQWPGRWH	NTX430388	0.0	1
SDDSFTDDLNKRPD	NTX496322	0.0	1
SDDSFTDDLNKRPDQRFSFG	NTX496322	0.0	1
SDSVYVIDKIIRPV	NTX135011	0.0	1
SDSVYVIDKIIRPVL	NTX135011	0.0	1
SGVGSETNDFGNGGTTTYYSDGSGTHT	NTX378014	0.0	1
TDEELAEYEKM	NTX56407	0.0	1
TQEKEQIKTLNNRF	NTX577282	0.0	1
TQEKEQIKTLNNRFAS	NTX577282	0.0	1
TVNIYPSPKPEIL	NTX51254	0.0	1
VLQYLPIYNTKFKQD	NTX468692	0.0	1
VNYYCRPDFGP	NTX627879	0.0	1
WVPTWSRATNKCRA	NTX512262	0.0	1
YLPIYNTKFKQD	NTX468692	0.0	1
LATKIIPSLFR	NTX134676	0.0017064846	12
ATKIIPSLFRR	NTX134676	0.0017064846	8
LFYHVCRCY	NTX359850	0.0017064846	7
LNKRPDQRFSF	NTX496322	0.0017064846	6
SFGLGKRPDQRFAF	NTX496322	0.0017064846	4
SFTDDLNKRPDQRFSF	NTX496322	0.0017064846	3
YAVPEGTLRT	NTX444153	0.0017064846	2
DSVYVIDKIIRPVLKDD	NTX135011	0.0017064846	1
FDPTSEAGAGRFDSVSAAGAGRF	NTX479826	0.0017064846	1
FEYKYSFKGPY	NTX335822	0.0017064846	1
FMVRLRNPWGGH	NTX189949	0.0017064846	1
GSVALTSENFDSHVGNHE	NTX629813	0.0017064846	1
HDDNGHFTNDWVVRITGGN	NTX487637	0.0017064846	1
IIPSLFRRKNQ	NTX134676	0.0017064846	1
LKDDDEYYLLEIDGKKGF	NTX51254	0.0017064846	1
TDDLNKRPDQRF	NTX496322	0.0017064846	1
TYINVSSLPGVNPPKPF	NTX271896	0.0017064846	1
YEPLTYNHEIVHQ	NTX136251	0.0017064846	1
NIMSFCDRED	NTX359850 NTX583552	0.0017064846 0.0017064846	6
LEFPNKGDKCEV	NTX413483 NTX413485	0.0017064846 0.0017064846	4
WCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0017064846 0.0017064846	4
YPSNCREEPGTGLYPDCCNKVVCG	NTX83486 NTX83487	0.0017064846 0.0017064846	4
FAFDDSKRYDQRFAF	NTX163968 NTX163969	0.0017064846 0.0017064846	2
FNPALEAGAGRFNPTSEAGA	NTX374064 NTX374066	0.0017064846 0.0017064846	2
KAVGKVIPELNGKLTGM	NTX270040 NTX534433	0.0017064846 0.0017064846	2
SRNDDIEEPFIKKT	NTX384947 NTX496574 NTX496576	0.0017064846 0.0017064846 0.0017064846	28
EYGDITGSGKDQDYWSS	NTX428770 NTX428772 NTX428775	0.0017064846 0.0017064846 0.0017064846	9
SEMESSIRDILT	NTX428770 NTX428772 NTX428775	0.0017064846 0.0017064846 0.0017064846	9
KIREEYPDRIM	NTX214579 NTX324164 NTX424777	0.0017064846 0.0017064846 0.0017064846	3
WISKQEYDESGPSIVHRKCF	NTX258811 NTX480808 NTX480845	0.0017064846 0.0017064846 0.0017064846	3
NDIVEEPFK	NTX496567 NTX496574 NTX496575 NTX496576	0.0017064846 0.0017064846 0.0017064846 0.0017064846	36
SRNDDIEEPFIKK	NTX384939 NTX384947 NTX496574 NTX496576	0.0017064846 0.0017064846 0.0017064846 0.0017064846	24
DIEEPFIKKSA	NTX384939 NTX384947 NTX496574 NTX496576	0.0017064846 0.0017064846 0.0017064846 0.0017064846	16
LRNDIVEEPFK	NTX496567 NTX496574 NTX496575 NTX496576	0.0017064846 0.0017064846 0.0017064846 0.0017064846	8
FNPTSEAGAGRFDPAS	NTX374059 NTX374061 NTX485691 NTX485694	0.0017064846 0.0017064846 0.0017064846 0.0017064846	4
LLLPGELAKHAVSEGTKAVTKY	NTX336196 NTX336206 NTX448011 NTX464600 NTX584434	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	10
MDVVYALKRQGR	NTX132749 NTX132751 NTX132752 NTX652442 NTX653517	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	5
STELLIRKLPFQRLV	NTX213988 NTX412398 NTX412401 NTX448016 NTX448019 NTX658946	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	42
KSYELPDGQVITIGNERFRCPEAL	NTX38478 NTX480808 NTX480816 NTX480817 NTX592828 NTX592829	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	12
TMKIKIIAPPERKY	NTX102642 NTX24145 NTX244697 NTX38477 NTX38478 NTX480808 NTX480845 NTX506496	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	48
LVVDNGSGMCKAGFAGDDAPR	NTX148639 NTX148652 NTX148683 NTX148684 NTX592806 NTX592809 NTX592810 NTX592811 NTX592814	0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846 0.0017064846	9
LACWCEGLPDDKTWKS	NTX41956 NTX41957 NTX41959	0.003407155 0.003407155 0.003407155	6
QPPPAPVYPRQF	NTX462752	0.0050167223	4
FTDDLNKRPDQRFSF	NTX496322	0.0050167223	1
VFDETADKLTKEFPEPGKL	NTX629813	0.0050167223	1
DNGYLLDKYTG	NTX434966 NTX434967	0.0050167223 0.0050167223	8
LFNDPLKEKEY	NTX334555 NTX334556	0.0050167223 0.0050167223	4
GRFDPSAGRFDPVSAAGAGRFEAGA	NTX485692 NTX485700	0.0050167223 0.0050167223	2
DIEEPFIKKT	NTX384947 NTX496574 NTX496576	0.0050167223 0.0050167223 0.0050167223	20
FNSWNDKFRP	NTX428770 NTX428772 NTX428775	0.0050167223 0.0050167223 0.0050167223	9
AAGAGRFDPVSAAGAGR	NTX591829 NTX591831 NTX591832	0.0050167223 0.0050167223 0.0050167223	3
GRFDPSSEAGAGRF	NTX374070 NTX485681 NTX485692 NTX485700	0.0050167223 0.0050167223 0.0050167223 0.0050167223	4
STELLIRKLPFQRL	NTX213988 NTX412398 NTX412401 NTX448016 NTX448019 NTX658946	0.0050167223 0.0050167223 0.0050167223 0.0050167223 0.0050167223 0.0050167223	18
YPSNCREEPGTGLYPDCCNKVVCGE	NTX83486 NTX83487	0.006677796 0.006677796	6
GYGEMVLDLEKQHCE	NTX549633	0.008143323	41
KTLNNRFASFIDKVRF	NTX577282	0.008143323	4
WKLACFCQGA	NTX323335	0.008143323	3
YAPYDYREEGHLT	NTX112170	0.008143323	3
FFGHLFKLA	NTX134676	0.008143323	2
LFKAVANDIEELSK	NTX56407	0.008143323	2
DDIEEPFIKKN	NTX384939	0.008143323	1
RTQEKEQIKTLNNRFAS	NTX577282	0.008143323	1
TVRTQEKEQIKTLNNRFA	NTX577282	0.008143323	1
EYCNDLCTKNGAKSGYCQ	NTX49728 NTX49729	0.008143323 0.008143323	2
KIREEYPDRIMN	NTX324164 NTX424777	0.008143323 0.008143323	2
ITEYDNRPFC	NTX361043 NTX584753 NTX584754	0.008143323 0.008143323 0.008143323	3
YASAVISKSNL	NTX361043 NTX584753 NTX584754	0.008143323 0.008143323 0.008143323	3
DIEEPFIKKS	NTX384939 NTX384947 NTX496574 NTX496576	0.008143323 0.008143323 0.008143323 0.008143323	4
FDPVSAAGAGFDRFDPVPAAE	NTX374058 NTX374063 NTX374072 NTX485687 NTX485696	0.008143323 0.008143323 0.008143323 0.008143323 0.008143323	5
LATKIIPSLFRR	NTX134676	0.009661836	4
SGGSYGYCWTWGLACWCEGLPDDKT	NTX41956	0.009661836	2
ESCWPNMLCCFAQFRCICHVNSCQYTNK	NTX135801	0.009661836	1
ADGPTGTLQEIR	NTX178649 NTX623208	0.009661836 0.009661836	2
FRTMWERYCRGVNAI	NTX4281 NTX4282	0.009661836 0.009661836	2
VRDAYIAKPHN	NTX423614 NTX535299 NTX83902	0.009661836 0.009661836 0.009661836	3
NDDIEEPFIKKTAYVTGGRS	NTX384947 NTX496574	0.0 0.0	404
SDKFPFENEFPK	NTX98053 NTX98054	0.0 0.0	58
NDDIEEPFIKKTAY	NTX384947 NTX496574	0.0 0.0	56
DDIEEPFIKKTAYVTGGRS	NTX384947 NTX496574	0.0 0.0	54
GCWCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	48
DIEEPFIKKTAYVTGGRS	NTX384947 NTX496574	0.0 0.0	42
NDDIEEPFIKKTAYV	NTX384947 NTX496574	0.0 0.0	38
CWCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	34
NDDIEEPFIKKTANVTGGRS	NTX496574 NTX496576	0.0 0.0	32
IELPDNVPIRVP	NTX49728 NTX49729	0.0 0.0	26
IELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	26
LNIPYFPKTISS	NTX584753 NTX584754	0.0 0.0	24
RLNIPYFPKTISS	NTX584753 NTX584754	0.0 0.0	22
CIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	20
LLEFPNKGDKCEV	NTX413483 NTX413485	0.0 0.0	20
SDKFPFENEFPKF	NTX98053 NTX98054	0.0 0.0	20
EYVSENFRKFPN	NTX305063 NTX305064	0.0 0.0	18
FEPMDQAGAGR	NTX374070 NTX485681	0.0 0.0	18
IELPDNVPIRVPGKC	NTX49728 NTX49729	0.0 0.0	18
YGNGCWCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	18
SRNDDIEEPFIKKTAY	NTX384947 NTX496574	0.0 0.0	16
DDIEEPFIKKTAY	NTX384947 NTX496574	0.0 0.0	14
EYGTDNAFGQHGYDNS	NTX541125 NTX541126	0.0 0.0	14
LLEFPNKGDKCE	NTX413483 NTX413485	0.0 0.0	14
FNPTSEAGAGRFDPASAAGAGR	NTX374059 NTX485691	0.0 0.0	12
NDDIEEPFIKKTAN	NTX496574 NTX496576	0.0 0.0	12
DIEEPFIKKTAY	NTX384947 NTX496574	0.0 0.0	10
FKNKYEDEINKRA	NTX465514 NTX577282	0.0 0.0	10
GLDEKVLEDEGLGERWENEN	NTX662347 NTX662348	0.0 0.0	10
GRDAYIAQNYNCVYH	NTX83904 NTX83906	0.0 0.0	10
LACWCDDIHN	NTX311250 NTX311253	0.0 0.0	10
LFLNKNDNQPIKE	NTX315967 NTX315968	0.0 0.0	10
LLEFPNKGDKC	NTX413483 NTX413485	0.0 0.0	10
NEYCNDLCTKNGAKSGYCQ	NTX49728 NTX49729	0.0 0.0	10
AENLRYWNLITGLR	NTX334555 NTX334556	0.0 0.0	8
DKFPFENEFPK	NTX98053 NTX98054	0.0 0.0	8
FDPASAAGAGRFDPTLEAGAGRFNPALEAGAGR	NTX591828 NTX591830	0.0 0.0	8
IELPDNVPIRVPG	NTX49728 NTX49729	0.0 0.0	8
KLACWCDDIHN	NTX311250 NTX311253	0.0 0.0	8
NEYCNDLCTKNGA	NTX49728 NTX49729	0.0 0.0	8
SAELNNLDIWNEDRKIS	NTX644816 NTX644819	0.0 0.0	8
SSEINSLDIPNEDRKIS	NTX644817 NTX644818	0.0 0.0	8
YPSNCREEPGTGLYPDCCNKVVCGEEE	NTX83486 NTX83487	0.0 0.0	8
AACYSSDCRVKCVAM	NTX178644 NTX178646	0.0 0.0	6
ACWCIDLPDKVPIR	NTX602037 NTX602038	0.0 0.0	6
AFDDSKRYDQRFAF	NTX163968 NTX163969	0.0 0.0	6
ASYDFSSNDPFPYPR	NTX523065 NTX523066	0.0 0.0	6
EIERPSHRILY	NTX365400 NTX477121	0.0 0.0	6
ELPDNVPIRVP	NTX49728 NTX49729	0.0 0.0	6
FNPTSEAGAGRFDPASAAGAG	NTX374059 NTX485691	0.0 0.0	6
GKYGNGCWCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	6
GRYGNACWCIDLPDKVPIR	NTX602037 NTX602038	0.0 0.0	6
IEEPFIKKTAYVTGGRS	NTX384947 NTX496574	0.0 0.0	6
IELPDNVPIR	NTX49728 NTX49729	0.0 0.0	6
LEEYTELFNDPLKEKEY	NTX334555 NTX334556	0.0 0.0	6
LEPAGDVIADDFTIVHGDNGEKDHT	NTX91255 NTX91256	0.0 0.0	6
NGCWCIELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	6
SMDGDMEDYMKR	NTX163968 NTX163969	0.0 0.0	6
SRNDDIEEPFIKKTAYVTGGRS	NTX384947 NTX496574	0.0 0.0	6
VINNESCNSECKIRGGY	NTX434966 NTX434967	0.0 0.0	6
YPSNCREEPGTGLYPDCCNKVVCGEE	NTX83486 NTX83487	0.0 0.0	6
ATVFYPSNCREEPGTGLYPDCCNKVVCGEEE	NTX83486 NTX83487	0.0 0.0	4
DIEEPFIKKTANVTGGRS	NTX496574 NTX496576	0.0 0.0	4
ELPDNVPIRVPG	NTX49728 NTX49729	0.0 0.0	4
ELPDNVPIRVPGKC	NTX49728 NTX49729	0.0 0.0	4
FKNKYEDEINKRAA	NTX465514 NTX577282	0.0 0.0	4
FNDPLKEKEYAENL	NTX334555 NTX334556	0.0 0.0	4
ICSMSKFGNC	NTX85997 NTX86002	0.0 0.0	4
IEEPFIKKTAY	NTX384947 NTX496574	0.0 0.0	4
LWGNDFCDKVCKKSGGS	NTX41956 NTX41958	0.0 0.0	4
LWGNEGCNKECKGFGA	NTX41959 NTX41960	0.0 0.0	4
SDKFPFENEFPKFFYSDLH	NTX98053 NTX98054	0.0 0.0	4
SFVPSSKKESPFAY	NTX224662 NTX3995	0.0 0.0	4
SGIDGVCEGDSGGPLVVR	NTX260492 NTX260493	0.0 0.0	4
SMDGDMEDYMK	NTX163968 NTX163969	0.0 0.0	4
SMDGDMEDYMKRRFS	NTX163968 NTX163969	0.0 0.0	4
SPLSKELRQKYNVR	NTX212167 NTX522813	0.0 0.0	4
TELFNDPLKEKEY	NTX334555 NTX334556	0.0 0.0	4
YTELFNDPLKEKEYAENL	NTX334555 NTX334556	0.0 0.0	4
ACWCEGLPAEKT	NTX262119 NTX262120	0.0 0.0	2
ADGPTGTLQEIRKKWGA	NTX178649 NTX623208	0.0 0.0	2
AVSMDNCSRTGVCMNTCHENI	NTX213008 NTX213009	0.0 0.0	2
CEGLPDNETWKYESNTCGS	NTX41958 NTX41960	0.0 0.0	2
DDSKRYDQRFAF	NTX163968 NTX163969	0.0 0.0	2
DNGYLLDKYTGCKVW	NTX434966 NTX434967	0.0 0.0	2
EEEHCESWICRA	NTX177808 NTX177809	0.0 0.0	2
ELPDNVPIRVPGKCH	NTX49728 NTX49729	0.0 0.0	2
EWSDPSVQHDIKYFPFK	NTX94322 NTX94323	0.0 0.0	2
EYGTDNAFGQHGYDNSL	NTX541125 NTX541126	0.0 0.0	2
FDPASEAGAGRFDPVSAAGAGRFDPVLEAGAGR	NTX479827 NTX591828	0.0 0.0	2
FDPTLEAGAGRFNPALEAGAGRFDPTSEAGAGR	NTX591829 NTX591832	0.0 0.0	2
LEPAGDVIADDFTIVHGDNGEKD	NTX91255 NTX91256	0.0 0.0	2
LEPAGDVIADDFTIVHGDNGEKDHTF	NTX91255 NTX91256	0.0 0.0	2
LGEHKLNDDNDGARPVDY	NTX317140 NTX317142	0.0 0.0	2
LGPILDSNKEFYFIHQAVPHA	NTX523065 NTX523066	0.0 0.0	2
LGQIFEDFYHLQH	NTX546099 NTX546100	0.0 0.0	2
LLEFPNKGDKCEVK	NTX413483 NTX413485	0.0 0.0	2
LWGNDFCDKVCKKSGG	NTX41956 NTX41958	0.0 0.0	2
LWGNEGCNKECKGFG	NTX41959 NTX41960	0.0 0.0	2
MLEKSDEDLEKRW	NTX249702 NTX249703	0.0 0.0	2
NACWCIDLPDKVPIR	NTX602037 NTX602038	0.0 0.0	2
NSIMKCDVDIRKDLYA	NTX38478 NTX480808	0.0 0.0	2
NSIMKCDVDIRKDLYAN	NTX38478 NTX480808	0.0 0.0	2
QSIYPLHDVYVRKVK	NTX227449 NTX427843	0.0 0.0	2
RLNIPYFPKT	NTX584753 NTX584754	0.0 0.0	2
SGIDGVCEGDSGGPLVVRRGTEY	NTX260492 NTX260493	0.0 0.0	2
SIPIEIRNKIED	NTX224662 NTX3995	0.0 0.0	2
SMDGDMEDYMKRR	NTX163968 NTX163969	0.0 0.0	2
SPLSKELRQKYNVRS	NTX212167 NTX522813	0.0 0.0	2
SVTDDNELIPR	NTX334429 NTX334430	0.0 0.0	2
VEIIANDQGNRITPS	NTX94322 NTX94323	0.0 0.0	2
VINNESCNSECKI	NTX434966 NTX434967	0.0 0.0	2
WGNEGCNKECKGFGA	NTX41959 NTX41960	0.0 0.0	2
WLDKWDGDSKRM	NTX249702 NTX249703	0.0 0.0	2
ARNEYCNDLCTKNGAKSGYCQ	NTX49728 NTX49728 NTX49729 NTX49729	0.0 0.009661836 0.0 0.009661836	6
DSEMESSIRDILT	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	123
NDDIEEPFIKKT	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	112
NDDIEEPFIKKTA	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	96
LKDDMTDFNSWNDKFRP	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	66
WCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	45
ACWCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	42
CWCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	36
KQEYDESGPSIVHRKCF	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	36
LDEIDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	36
SRNDDIEEPFIKKTA	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	36
SVLDDLNNQLDNLH	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	36
VVGGSDADKNAFRYASA	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	36
FDPASAAGAGRFDPVSAAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	33
IFNKENYPH	NTX361043 NTX472849 NTX584754	0.0 0.0 0.0	33
AKNDDVEIVDHNFF	NTX144303 NTX144307 NTX144311	0.0 0.0 0.0	30
AKNDDVEIVDHNFFRF	NTX144303 NTX144307 NTX144311	0.0 0.0 0.0	30
EIDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	30
FDPASVAGAGR	NTX374070 NTX485681 NTX485692	0.0 0.0 0.0	30
LLDDDLKEELEEN	NTX201404 NTX310969 NTX91175	0.0 0.0 0.0	30
VVGGSDADKNAFRY	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	30
DDIEEPFIKKT	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	28
DIEEPFIKKTA	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	28
GLACWCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	27
YACPVIEKWCEDHCAA	NTX372384 NTX484016 NTX484018	0.0 0.0 0.0	27
DDIEEPFIKKTA	NTX384947 NTX496574 NTX496576	0.0 0.0 0.0	24
FDPASEAGAGRFDPVSAAGAGR	NTX43000 NTX479827 NTX591828	0.0 0.0 0.0	24
LACWCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	21
FDPASVAGAGRFD	NTX374070 NTX485681 NTX485692	0.0 0.0 0.0	18
TDMAMCGATLITDRH	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	18
VDVPITSTEECQKELVHT	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	18
VIEKWCEDHCAA	NTX372384 NTX484016 NTX484018	0.0 0.0 0.0	18
WCEGLPDDKTWKS	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	18
ADPAKNDDVEIVDHNFFRF	NTX144303 NTX144307 NTX144311	0.0 0.0 0.0	15
EYGDITGSGKDQDY	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	15
FDPVSAAGAGRFDPTLEAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	15
IDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	15
SYCSLGIGNSEECLTKQ	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	15
YADPAKNDDVEIVDHNFFRF	NTX144303 NTX144307 NTX144311	0.0 0.0 0.0	15
DEIDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	12
FNPALEAGAGRFDPTSEAGAGR	NTX479826 NTX591829 NTX591832	0.0 0.0 0.0	12
ITEYDNRPFCITDMDFSNIKD	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	12
VDVPITSTEECQKE	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	12
VVGGSDADKNAFRYA	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	12
WCEGLPDDKTWKSESN	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	12
DNRPFCITDMDFSNIKD	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
EYGDITGSGKDQDYWSSGRTP	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	9
FDPAAEAGAGRFD	NTX374059 NTX485691 NTX485694	0.0 0.0 0.0	9
FDPSSEAGAGRFDPASVAGAGR	NTX374070 NTX485681 NTX485692	0.0 0.0 0.0	9
FDPTSEAGAGRFDPVSAAGAGR	NTX591828 NTX591829 NTX591832	0.0 0.0 0.0	9
FNPALEAGAGRFDPASEAGAGR	NTX374058 NTX374063 NTX591828	0.0 0.0 0.0	9
ISKQEYDESGPSIVH	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	9
IYEELGEKYKNRNDIVIA	NTX235912 NTX235914 NTX235916	0.0 0.0 0.0	9
LITDRHVITAAHC	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
LSTFQQMWISKQEYDESGPSIVHRKCF	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	9
NDDVINYIREIE	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
PASAAGAGRFDPVSAAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	9
SCVLNLPGMDCEYGDITGSGKDQDY	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	9
SIMKCDVDIRKDLYAN	NTX38478 NTX480808 NTX480817	0.0 0.0 0.0	9
SKQEYDESGPSIVH	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	9
SYCSLGIGNSEEC	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	9
VVGGSDADKNAFRYAS	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
VVGGSDADKNAFRYASAVIS	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
VVGGSDADKNAFRYASAVISKSNL	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	9
WCEGLPDDKTWKSE	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	9
YACPVIEKWCEDHC	NTX372384 NTX484016 NTX484018	0.0 0.0 0.0	9
YNIEREEELKE	NTX201404 NTX310969 NTX91175	0.0 0.0 0.0	9
EECNAILEENKFY	NTX438454 NTX438456 NTX438457	0.0 0.0 0.0	6
FDPASAAGAGRFDPVSAAGAGRFD	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	6
FDPASAAGAGRFDPVSAAGAGRFDPTLEAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	6
FDPVSAAGAGRFDPTLEAGAGRFNPALEAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	6
FKNKYEDEINKR	NTX283712 NTX465514 NTX577282	0.0 0.0 0.0	6
FLILVGTKWCGAGN	NTX644816 NTX644818 NTX644819	0.0 0.0 0.0	6
FVQAENYNVACGIVSFGRRCAL	NTX361043 NTX472849 NTX584754	0.0 0.0 0.0	6
IFSKSKMPIFPHEN	NTX134688 NTX134689 NTX244706	0.0 0.0 0.0	6
ISKQEYDESGPSIVHRKCF	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	6
KQEYDESGPSIVH	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	6
LSLMKDVNNLTYLIK	NTX134688 NTX134689 NTX244706	0.0 0.0 0.0	6
MDATANELEHTKINSFPTIKLY	NTX235912 NTX235914 NTX235916	0.0 0.0 0.0	6
MKDVNNLTYLIK	NTX134688 NTX134689 NTX244706	0.0 0.0 0.0	6
PVSAAGAGRFDPTLEAGAGR	NTX591829 NTX591831 NTX591832	0.0 0.0 0.0	6
QELDEIDKSGFGGF	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	6
SADCRPRIFN	NTX361043 NTX472849 NTX584754	0.0 0.0 0.0	6
SCVLNLPGMDCEYGDITGSGKDQDYWSSGRTP	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	6
SVLDDLNNQLDNLHK	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	6
SYCSLGIGNSEECLTKQL	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	6
TDMAMCGATLITDRHVITAAHCLYGKGS	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	6
VEDFKNKYEDEINK	NTX283712 NTX465514 NTX577282	0.0 0.0 0.0	6
VIEKWCEDHC	NTX372384 NTX484016 NTX484018	0.0 0.0 0.0	6
VITAAHCLYGKGS	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	6
VVGGSDADKNAFRYASAV	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	6
WGLACWCEGLPDDKT	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	6
WNTITEYDNRPFCITDMDFSNIKD	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	6
YDNRPFCITDMDFSNIKD	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	6
ARSVLDDLNNQLDNLH	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	3
DPAKNDDVEIVDHNFFRF	NTX144303 NTX144307 NTX144311	0.0 0.0 0.0	3
ELDEIDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	3
FDPAAEAGAGRFDP	NTX374059 NTX485691 NTX485694	0.0 0.0 0.0	3
FDPASVAGAGRF	NTX374070 NTX485681 NTX485692	0.0 0.0 0.0	3
FDPASVAGAGRFDPTLEAGAGR	NTX374070 NTX485681 NTX485692	0.0 0.0 0.0	3
FKNKYEDEINK	NTX283712 NTX465514 NTX577282	0.0 0.0 0.0	3
IEEQFPTHEPYQEER	NTX164951 NTX164952 NTX164953	0.0 0.0 0.0	3
IFPHENKESLFPK	NTX134688 NTX134689 NTX244706	0.0 0.0 0.0	3
ISKQEYDESGPSIVHR	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	3
ITDMDFSNIKD	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	3
KESLFPKYKLQY	NTX134688 NTX134689 NTX244706	0.0 0.0 0.0	3
KLACWCDNIHN	NTX311249 NTX311255 NTX512262	0.0 0.0 0.0	3
LACWCEGLPDDKTW	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	3
LAYPGTLCNKCM	NTX333875 NTX333876 NTX445670	0.0 0.0 0.0	3
LKDDMTDFNSWNDK	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	3
MDATANELEHTKINSFPTIKL	NTX235912 NTX235914 NTX235916	0.0 0.0 0.0	3
NDDVINYIRE	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	3
QELDEIDKSGFGGFH	NTX334743 NTX334744 NTX334745	0.0 0.0 0.0	3
SCVLNLPGMDCEYGDITGSGKDQD	NTX428770 NTX428772 NTX428775	0.0 0.0 0.0	3
STFQQMWISKQEYDESGPSIVHRKCF	NTX258811 NTX480808 NTX480845	0.0 0.0 0.0	3
TDMAMCGATLITDRHVITA	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	3
TDMAMCGATLITDRHVITAAHC	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	3
VDVPITSTEECQ	NTX361043 NTX584753 NTX584754	0.0 0.0 0.0	3
VIEKWCEDHCA	NTX372384 NTX484016 NTX484018	0.0 0.0 0.0	3
VIHPTIHTLDGRE	NTX224320 NTX3648 NTX3649	0.0 0.0 0.0	3
WCEGLPDDKTW	NTX41956 NTX41957 NTX41959	0.0 0.0 0.0	3
YSPVIDYDKLKNERPEF	NTX558144 NTX558145 NTX558147	0.0 0.0 0.0	3
NDDIEEPFIKKSAYVTGGRS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	436
NDDIEEPFIKK	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	184
NDDIEEPFIKKSA	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	120
NDDIEEPFIKKS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	116
TPYPVNCKTDRDCVMCGLGISCKNGYCQGCT	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	96
NDDIEEPFIKKSAY	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	92
DDIEEPFIKKSAYVTGGRS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	80
DIEEPFIKKSAYVTGGRS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	68
NDDIEEPFIK	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	68
NDIVEEPFKKKTAY	NTX496567 NTX496574 NTX496575 NTX496576	0.0 0.0 0.0 0.0	48
DHDGDDHNEELEEDVKQE	NTX565257 NTX565258 NTX565259 NTX565261	0.0 0.0 0.0 0.0	44
SRNDDIEEPFIK	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	36
LSDDKCVKIEY	NTX494486 NTX494487 NTX494488 NTX494489	0.0 0.0 0.0 0.0	32
FDPAAEAGAGR	NTX374059 NTX485681 NTX485691 NTX485694	0.0 0.0 0.0 0.0	28
NDIVEEPFKKKTAYVTGGRS	NTX496567 NTX496574 NTX496575 NTX496576	0.0 0.0 0.0 0.0	28
FDPSSEAGAGRFD	NTX374070 NTX485681 NTX485692 NTX485700	0.0 0.0 0.0 0.0	24
YPVNCKTDRDCVMCGLGISCKNGYCQGCT	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	24
DDIEEPFIKKS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	20
DDIEEPFIKKSAY	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	20
DIEEPFIKKSAY	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	20
GRFDPSSEAGAGRFD	NTX374070 NTX485681 NTX485692 NTX485700	0.0 0.0 0.0 0.0	20
GTPYPVNCKTDRDCVMCGLGISCKNGYCQGCT	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	20
IEEPFIKKSAYVTGGRS	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	20
TPYPVNCKTDRD	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	20
TPYPVNCKTDRDCVM	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	20
DDIEEPFIKKSA	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	16
DEGSLDCPDSDRY	NTX311050 NTX311051 NTX311052 NTX311053	0.0 0.0 0.0 0.0	16
FDTALAGGAFGETKE	NTX373661 NTX373662 NTX485295 NTX485296	0.0 0.0 0.0 0.0	16
SRNDDIEEPFIKKSAY	NTX384939 NTX384947 NTX496574 NTX496576	0.0 0.0 0.0 0.0	16
CGLGISCKNGYCQGCT	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	12
DDEPRECCPSVMEINEPR	NTX218921 NTX328346 NTX328347 NTX328348	0.0 0.0 0.0 0.0	12
FDPAAEAGAGRF	NTX374059 NTX485681 NTX485691 NTX485694	0.0 0.0 0.0 0.0	12
FDPSSEAGAGRF	NTX374070 NTX485681 NTX485692 NTX485700	0.0 0.0 0.0 0.0	12
LPDDKCVKIEY	NTX287294 NTX67369 NTX67370 NTX67371	0.0 0.0 0.0 0.0	12
NDIVEEPFKK	NTX496567 NTX496574 NTX496575 NTX496576	0.0 0.0 0.0 0.0	12
NDIVEEPFKKKTA	NTX496567 NTX496574 NTX496575 NTX496576	0.0 0.0 0.0 0.0	12
CVMCGLGISCKNGYCQGCT	NTX371729 NTX371731 NTX371733 NTX483383	0.0 0.0 0.0 0.0	8
FDPSSEAGAGRFDPAS	NTX374070 NTX485681 NTX485692 NTX485700	0.0 0.0 0.0 0.0	8
FNPTSEAGAGRFDPVSAAGAGRFEAGAGRFNPTSEAGA	NTX374059 NTX374061 NTX485691 NTX485694	0.0 0.0 0.0 0.0	8

Download the peptide summary

Furthermore, you can get more detailed PSM (peptide-spectrum match) information from the link below:

Download the PSM summary

3 Annotation

This chapter aims to explore the sequence motifs and potential function of the peptides via serveral different tools.

3.1 MSA

Multiple Sequence Alignment (MSA) is generally the alignment of three or more biological sequences (protein or nucleic acid) with similar length. From the output, homology can be inferred and the evolutionary relationships between the sequences cand be studied.

Click here to the multiple sequence alignment viewer

3.2 SignalP

The secretory signal peptide is a ubiquitous proteinsorting signal that targets its passenger protein for translocation across the endoplasmic reticulum membrane in eukaryotes and the cytoplasmic membrane in prokaryotes. In this section, the signalP 4.1 server, which is designed to discriminate between signal peptides and transmembrane regions, is utilized for predicting the presence and location of signal peptide cleavage sites in amino acid sequences from different organisms.

ID	D	isSignal	Dmaxcut	Networks-used	Cmax	Ymax	Smax
NTX4281	0.104	N	0.45	SignalP-noTM	0.114	0.108	0.113
NTX13418	0.889	Y	0.45	SignalP-noTM	0.803	0.857	0.96
NTX14137	0.104	N	0.45	SignalP-noTM	0.108	0.106	0.118
NTX24145	0.099	N	0.45	SignalP-noTM	0.114	0.104	0.109
NTX28979	0.652	Y	0.45	SignalP-noTM	0.613	0.643	0.87
NTX38478	0.103	N	0.45	SignalP-noTM	0.117	0.107	0.139
NTX41956	0.12	N	0.45	SignalP-noTM	0.126	0.125	0.166
NTX41957	0.127	N	0.45	SignalP-noTM	0.134	0.133	0.186
NTX41958	0.122	N	0.45	SignalP-noTM	0.13	0.128	0.167
NTX41959	0.127	N	0.45	SignalP-noTM	0.125	0.13	0.197
NTX42998	0.113	N	0.45	SignalP-noTM	0.133	0.119	0.133
NTX43000	0.124	N	0.45	SignalP-noTM	0.172	0.139	0.159
NTX49728	0.101	N	0.45	SignalP-noTM	0.11	0.105	0.109
NTX51254	0.331	N	0.5	SignalP-TM	0.445	0.407	0.647
NTX56407	0.103	N	0.45	SignalP-noTM	0.109	0.107	0.115
NTX83486	0.312	N	0.5	SignalP-TM	0.588	0.343	0.468
NTX83904	0.432	N	0.5	SignalP-TM	0.268	0.405	0.771
NTX83905	0.427	N	0.5	SignalP-TM	0.284	0.403	0.729
NTX85997	0.287	N	0.5	SignalP-TM	0.213	0.28	0.685
NTX91175	0.274	N	0.5	SignalP-TM	0.297	0.272	0.401
NTX91255	0.102	N	0.45	SignalP-noTM	0.131	0.112	0.123
NTX94322	0.698	Y	0.45	SignalP-noTM	0.555	0.643	0.859
NTX98053	0.101	N	0.45	SignalP-noTM	0.111	0.104	0.166
NTX102642	0.099	N	0.45	SignalP-noTM	0.107	0.103	0.144
NTX104669	0.552	Y	0.5	SignalP-TM	0.678	0.629	0.86
NTX112170	0.171	N	0.5	SignalP-TM	0.262	0.185	0.29
NTX132748	0.099	N	0.45	SignalP-noTM	0.11	0.103	0.108
NTX132749	0.099	N	0.45	SignalP-noTM	0.11	0.103	0.108
NTX134676	0.252	N	0.5	SignalP-TM	0.458	0.308	0.394
NTX134688	0.102	N	0.45	SignalP-noTM	0.106	0.107	0.123
NTX135011	0.593	Y	0.45	SignalP-noTM	0.445	0.534	0.853
NTX135801	0.42	N	0.45	SignalP-noTM	0.151	0.244	0.656
NTX136251	0.702	Y	0.45	SignalP-noTM	0.667	0.69	0.764
NTX142744	0.099	N	0.45	SignalP-noTM	0.109	0.104	0.118
NTX144303	0.684	Y	0.45	SignalP-noTM	0.298	0.506	0.946
NTX148639	0.102	N	0.45	SignalP-noTM	0.119	0.108	0.116
NTX154016	0.202	N	0.5	SignalP-TM	0.148	0.217	0.711
NTX163968	0.104	N	0.45	SignalP-noTM	0.112	0.107	0.123
NTX163969	0.104	N	0.45	SignalP-noTM	0.112	0.107	0.123
NTX164951	0.103	N	0.45	SignalP-noTM	0.108	0.108	0.12
NTX173892	0.122	N	0.45	SignalP-noTM	0.177	0.141	0.147
NTX177486	0.104	N	0.45	SignalP-noTM	0.11	0.108	0.118
NTX177808	0.23	N	0.5	SignalP-TM	0.335	0.281	0.52
NTX178644	0.454	N	0.5	SignalP-TM	0.212	0.369	0.813
NTX178649	0.663	Y	0.45	SignalP-noTM	0.288	0.482	0.91
NTX181117	0.11	N	0.45	SignalP-noTM	0.107	0.11	0.126
NTX186740	0.103	N	0.45	SignalP-noTM	0.112	0.11	0.123
NTX189949	0.1	N	0.45	SignalP-noTM	0.113	0.104	0.115
NTX201404	0.321	N	0.5	SignalP-TM	0.401	0.293	0.611
NTX212167	0.099	N	0.45	SignalP-noTM	0.109	0.103	0.111
NTX213008	0.871	Y	0.45	SignalP-noTM	0.696	0.802	0.989
NTX213988	0.256	N	0.45	SignalP-noTM	0.135	0.2	0.483
NTX214579	0.099	N	0.45	SignalP-noTM	0.113	0.104	0.117
NTX218921	0.274	N	0.45	SignalP-noTM	0.267	0.266	0.455
NTX223142	0.259	N	0.5	SignalP-TM	0.156	0.204	0.565
NTX224320	0.152	N	0.5	SignalP-TM	0.161	0.167	0.233
NTX224662	0.111	N	0.45	SignalP-noTM	0.108	0.115	0.144
NTX226741	0.127	N	0.45	SignalP-noTM	0.109	0.122	0.183
NTX227449	0.113	N	0.45	SignalP-noTM	0.133	0.12	0.125
NTX235909	0.101	N	0.45	SignalP-noTM	0.111	0.105	0.122
NTX235912	0.101	N	0.45	SignalP-noTM	0.109	0.105	0.12
NTX248037	0.116	N	0.45	SignalP-noTM	0.107	0.112	0.15
NTX249700	0.903	Y	0.45	SignalP-noTM	0.782	0.858	0.981
NTX249702	0.104	N	0.45	SignalP-noTM	0.106	0.105	0.118
NTX258811	0.12	N	0.45	SignalP-noTM	0.107	0.122	0.226
NTX260492	0.141	N	0.45	SignalP-noTM	0.149	0.147	0.18
NTX262119	0.809	Y	0.45	SignalP-noTM	0.638	0.74	0.973
NTX270040	0.12	N	0.45	SignalP-noTM	0.113	0.117	0.148
NTX271896	0.155	N	0.5	SignalP-TM	0.386	0.142	0.263
NTX283712	0.129	N	0.45	SignalP-noTM	0.109	0.125	0.168
NTX287294	0.114	N	0.45	SignalP-noTM	0.116	0.116	0.142
NTX305063	0.252	N	0.5	SignalP-TM	0.396	0.29	0.273
NTX311050	0.106	N	0.45	SignalP-noTM	0.112	0.108	0.128
NTX311249	0.137	N	0.45	SignalP-noTM	0.173	0.149	0.167
NTX311250	0.183	N	0.45	SignalP-noTM	0.134	0.164	0.256
NTX312243	0.359	N	0.45	SignalP-noTM	0.155	0.266	0.668
NTX315967	0.101	N	0.45	SignalP-noTM	0.107	0.103	0.114
NTX317140	0.109	N	0.45	SignalP-noTM	0.11	0.112	0.152
NTX323335	0.13	N	0.45	SignalP-noTM	0.125	0.128	0.174
NTX324164	0.099	N	0.45	SignalP-noTM	0.116	0.106	0.113
NTX333875	0.146	N	0.45	SignalP-noTM	0.115	0.138	0.215
NTX334429	0.143	N	0.5	SignalP-TM	0.124	0.144	0.245
NTX334555	0.103	N	0.45	SignalP-noTM	0.11	0.106	0.116
NTX334743	0.099	N	0.45	SignalP-noTM	0.107	0.103	0.113
NTX335822	0.263	N	0.5	SignalP-TM	0.157	0.235	0.533
NTX336196	0.107	N	0.45	SignalP-noTM	0.113	0.11	0.145
NTX352134	0.155	N	0.45	SignalP-noTM	0.167	0.157	0.244
NTX359850	0.766	Y	0.45	SignalP-noTM	0.692	0.735	0.946
NTX361043	0.274	N	0.45	SignalP-noTM	0.166	0.223	0.481
NTX365400	0.4	N	0.5	SignalP-TM	0.638	0.46	0.424
NTX366384	0.877	Y	0.45	SignalP-noTM	0.873	0.886	0.973
NTX371729	0.945	Y	0.45	SignalP-noTM	0.882	0.922	0.993
NTX372159	0.242	N	0.45	SignalP-noTM	0.179	0.227	0.45
NTX372384	0.103	N	0.45	SignalP-noTM	0.108	0.106	0.122
NTX373661	0.735	Y	0.45	SignalP-noTM	0.797	0.762	0.863
NTX374058	0.112	N	0.45	SignalP-noTM	0.133	0.119	0.145
NTX374059	0.105	N	0.45	SignalP-noTM	0.111	0.108	0.119
NTX374060	0.107	N	0.45	SignalP-noTM	0.11	0.107	0.139
NTX374063	0.107	N	0.45	SignalP-noTM	0.116	0.109	0.128
NTX374064	0.105	N	0.45	SignalP-noTM	0.112	0.108	0.125
NTX374070	0.119	N	0.45	SignalP-noTM	0.112	0.117	0.143
NTX378014	0.096	N	0.45	SignalP-noTM	0.106	0.101	0.107
NTX384939	0.102	N	0.45	SignalP-noTM	0.109	0.105	0.111
NTX384947	0.102	N	0.45	SignalP-noTM	0.109	0.105	0.112
NTX385699	0.282	N	0.45	SignalP-noTM	0.374	0.311	0.552
NTX407408	0.088	N	0.5	SignalP-TM	0.114	0.103	0.15
NTX413483	0.173	N	0.5	SignalP-TM	0.135	0.164	0.321
NTX416169	0.221	N	0.5	SignalP-TM	0.283	0.244	0.35
NTX416183	0.17	N	0.5	SignalP-TM	0.417	0.213	0.318
NTX423614	0.203	N	0.5	SignalP-TM	0.137	0.164	0.377
NTX428770	0.687	Y	0.45	SignalP-noTM	0.468	0.597	0.92
NTX430388	0.676	Y	0.45	SignalP-noTM	0.494	0.595	0.918
NTX431293	0.1	N	0.45	SignalP-noTM	0.108	0.105	0.121
NTX434966	0.343	N	0.5	SignalP-TM	0.406	0.376	0.506
NTX438454	0.101	N	0.45	SignalP-noTM	0.112	0.106	0.113
NTX444153	0.265	N	0.5	SignalP-TM	0.195	0.226	0.585
NTX462752	0.726	Y	0.5	SignalP-TM	0.55	0.665	0.88
NTX465514	0.285	N	0.45	SignalP-noTM	0.138	0.211	0.534
NTX468692	0.132	N	0.5	SignalP-TM	0.121	0.131	0.202
NTX479826	0.107	N	0.45	SignalP-noTM	0.111	0.108	0.135
NTX479827	0.114	N	0.45	SignalP-noTM	0.121	0.116	0.133
NTX485684	0.095	N	0.45	SignalP-noTM	0.11	0.1	0.106
NTX485692	0.101	N	0.45	SignalP-noTM	0.109	0.106	0.125
NTX487637	0.826	Y	0.45	SignalP-noTM	0.612	0.742	0.934
NTX487647	0.116	N	0.45	SignalP-noTM	0.129	0.121	0.143
NTX494486	0.099	N	0.45	SignalP-noTM	0.107	0.104	0.131
NTX496322	0.098	N	0.45	SignalP-noTM	0.109	0.102	0.104
NTX496567	0.749	Y	0.45	SignalP-noTM	0.574	0.681	0.901
NTX496574	0.748	Y	0.45	SignalP-noTM	0.577	0.682	0.9
NTX512262	0.123	N	0.45	SignalP-noTM	0.11	0.116	0.169
NTX519666	0.105	N	0.45	SignalP-noTM	0.116	0.11	0.139
NTX523065	0.147	N	0.5	SignalP-TM	0.127	0.151	0.283
NTX541125	0.105	N	0.45	SignalP-noTM	0.165	0.122	0.115
NTX546099	0.105	N	0.45	SignalP-noTM	0.11	0.105	0.117
NTX549633	0.094	N	0.45	SignalP-noTM	0.105	0.098	0.103
NTX558144	0.192	N	0.5	SignalP-TM	0.179	0.159	0.352
NTX565257	0.847	Y	0.45	SignalP-noTM	0.739	0.827	0.974
NTX574605	0.242	N	0.5	SignalP-TM	0.462	0.304	0.384
NTX577282	0.106	N	0.45	SignalP-noTM	0.109	0.105	0.116
NTX581382	0.524	Y	0.45	SignalP-noTM	0.168	0.319	0.895
NTX583217	0.099	N	0.45	SignalP-noTM	0.111	0.104	0.112
NTX584753	0.381	N	0.5	SignalP-TM	0.27	0.325	0.644
NTX591828	0.123	N	0.45	SignalP-noTM	0.112	0.123	0.181
NTX591829	0.141	N	0.45	SignalP-noTM	0.212	0.162	0.194
NTX592223	0.228	N	0.5	SignalP-TM	0.194	0.24	0.432
NTX596227	0.77	Y	0.45	SignalP-noTM	0.621	0.713	0.974
NTX597930	0.104	N	0.45	SignalP-noTM	0.118	0.108	0.122
NTX602037	0.108	N	0.45	SignalP-noTM	0.106	0.108	0.143
NTX602038	0.108	N	0.45	SignalP-noTM	0.107	0.108	0.143
NTX612360	0.099	N	0.45	SignalP-noTM	0.112	0.104	0.111
NTX624266	0.163	N	0.5	SignalP-TM	0.173	0.17	0.348
NTX627879	0.892	Y	0.45	SignalP-noTM	0.811	0.868	0.974
NTX629813	0.373	N	0.5	SignalP-TM	0.467	0.431	0.592
NTX637329	0.12	N	0.5	SignalP-TM	0.106	0.117	0.16
NTX644816	0.273	N	0.5	SignalP-TM	0.385	0.316	0.664
NTX644817	0.266	N	0.5	SignalP-TM	0.335	0.296	0.598
NTX660903	0.137	N	0.45	SignalP-noTM	0.16	0.145	0.189
NTX662347	0.11	N	0.45	SignalP-noTM	0.109	0.113	0.138

3.3 Venom annotation

Venoms are substances that are secreted by animals for either defensive or offensive purposes and that have a toxic or harmful effect on other animals. We annotated the endogenous peptides with an open-source and publicly accessible resource for researchers and other individuals interested in venom therapeutics (VenomKB,http://venomkb.org). In the following table,the venom entry related with an endogenous peptide will be merged into one group for presentation.

Venomkb_id	Percentage_identical	Expect_value	Venom_name	Venomkb_link
NTX14137
P4592344	100	0.0e+00	BmK-YA precursor	http://venomkb.org/P4592344
NTX41956
P4943114	91.379	0.0e+00	Toxin BmKITc	http://venomkb.org/P4943114
P3077021	86.207	0.0e+00	Anti-neuroexcitation peptide 2	http://venomkb.org/P3077021
P7736827	85.965	0.0e+00	Beta-insect depressant toxin LqhIT2	http://venomkb.org/P7736827
P1990136	85.965	0.0e+00	Insect toxin 2-53	http://venomkb.org/P1990136
P2415271	82.759	0.0e+00	Beta-insect depressant toxin BmKITa	http://venomkb.org/P2415271
NTX41957
P2415271	96.552	0.0e+00	Beta-insect depressant toxin BmKITa	http://venomkb.org/P2415271
P3077021	96.552	0.0e+00	Anti-neuroexcitation peptide 2	http://venomkb.org/P3077021
P3706610	96.552	0.0e+00	Depressant insect toxin BmK ITa1	http://venomkb.org/P3706610
P8255564	93.103	0.0e+00	Neurotoxin BmKAEP2	http://venomkb.org/P8255564
P0517870	96.552	0.0e+00	Toxin BmKIT3	http://venomkb.org/P0517870
NTX41958
P4943114	98.276	0.0e+00	Toxin BmKITc	http://venomkb.org/P4943114
P7331272	80	0.0e+00	Beta-insect depressant toxin BotIT4	http://venomkb.org/P7331272
P2906605	80	0.0e+00	Beta-insect depressant toxin BaIT2	http://venomkb.org/P2906605
P2923788	80	0.0e+00	Beta-insect depressant toxin BotIT5	http://venomkb.org/P2923788
NTX41959
P0314897	96.552	0.0e+00	Anti-neuroexcitation peptide 3	http://venomkb.org/P0314897
P1446795	96.552	0.0e+00	Depressant scorpion toxin BmKIM	http://venomkb.org/P1446795
P8255564	96.552	0.0e+00	Neurotoxin BmKAEP2	http://venomkb.org/P8255564
P5127212	94.828	0.0e+00	Beta-insect depressant toxin BmKITb	http://venomkb.org/P5127212
P6179056	94.828	0.0e+00	Beta-insect depressant toxin BmKIT4	http://venomkb.org/P6179056
NTX49728
P4787611	100	0.0e+00	Alpha-like toxin BmK-M1	http://venomkb.org/P4787611
P4952465	97.959	0.0e+00	Toxin BmKaTx16	http://venomkb.org/P4952465
P1496516	93.75	0.0e+00	Neurotoxin BmK-II	http://venomkb.org/P1496516
P3686529	95.833	0.0e+00	Alpha-like toxin BmK-M2	http://venomkb.org/P3686529
P6350894	85.417	0.0e+00	Alpha-like toxin Bom4	http://venomkb.org/P6350894
NTX83486
P3902032	100	0.0e+00	Venom peptide MmKTx1	http://venomkb.org/P3902032
NTX83904
P7310876	84.783	0.0e+00	Toxin BmKaIT1	http://venomkb.org/P7310876
P7984981	88.571	0.0e+00	Toxin BmKaTx17	http://venomkb.org/P7984981
P3593548	88.889	0.0e+00	Neurotoxin BmK AGP-SYPU1	http://venomkb.org/P3593548
P3727578	83.333	0.0e+00	Toxin Boma6d	http://venomkb.org/P3727578
P3214126	81.818	0.0e+00	Neurotoxin 5	http://venomkb.org/P3214126
NTX83905
P7820336	95.833	0.0e+00	Makatoxin-3	http://venomkb.org/P7820336
P2003384	87.5	0.0e+00	Alpha-toxin BmalphaTx47	http://venomkb.org/P2003384
P8379826	83.333	0.0e+00	Makatoxin-2	http://venomkb.org/P8379826
P4952465	85.417	0.0e+00	Toxin BmKaTx16	http://venomkb.org/P4952465
P4787611	85.417	0.0e+00	Alpha-like toxin BmK-M1	http://venomkb.org/P4787611
NTX85997
P6327864	100	0.0e+00	Potassium channel toxin alpha-KTx 15.2	http://venomkb.org/P6327864
P0728663	93.22	0.0e+00	Potassium channel toxin alpha-KTx 15.5	http://venomkb.org/P0728663
P2450445	91.525	0.0e+00	Potassium channel toxin alpha-KTx 15.4	http://venomkb.org/P2450445
P6545893	91.379	0.0e+00	Potassium channel toxin alpha-KTx 15.8	http://venomkb.org/P6545893
P5030120	88.136	0.0e+00	Potassium channel toxin alpha-KTx 15.7	http://venomkb.org/P5030120
NTX134676
P4376039	92.5	0.0e+00	Venom antimicrobial peptide-9	http://venomkb.org/P4376039
NTX178644
P7095662	100	0.0e+00	Potassium channel toxin alpha-KTx 19.1	http://venomkb.org/P7095662
NTX262119
P7736827	83.529	0.0e+00	Beta-insect depressant toxin LqhIT2	http://venomkb.org/P7736827
P1990136	82.353	0.0e+00	Insect toxin 2-53	http://venomkb.org/P1990136
P4338962	80.488	0.0e+00	Beta-insect depressant toxin LqqIT2	http://venomkb.org/P4338962
NTX311249
P9897492	96.364	0.0e+00	BmKBT-like peptide	http://venomkb.org/P9897492
P0420222	83.636	0.0e+00	Toxin BmKBT	http://venomkb.org/P0420222
NTX311250
P0420222	98.182	0.0e+00	Toxin BmKBT	http://venomkb.org/P0420222
P9897492	81.818	0.0e+00	BmKBT-like peptide	http://venomkb.org/P9897492
NTX323335
P2113503	86.667	6.0e-06	Beta-mammal/insect toxin Lqhb1	http://venomkb.org/P2113503
P0234296	86.667	7.1e-06	Neurotoxin MeuNaTx-6	http://venomkb.org/P0234296
P8482100	86.667	8.4e-06	Beta-toxin BmKAs1	http://venomkb.org/P8482100
NTX371729
P9849695	100	0.0e+00	Neurotoxin BmP08	http://venomkb.org/P9849695
P7368669	98.182	0.0e+00	Neurotoxin X-29S	http://venomkb.org/P7368669
P4131211	96.364	0.0e+00	Neurotoxin BmKX-A1-S31	http://venomkb.org/P4131211
NTX372159
P7454390	98.113	0.0e+00	Peptide BmKn1	http://venomkb.org/P7454390
P0029781	93.878	0.0e+00	Peptide BmKn2	http://venomkb.org/P0029781
P9988877	83.673	0.0e+00	Venom antimicrobial peptide-6	http://venomkb.org/P9988877
NTX372384
P6822073	92.105	0.0e+00	Potassium channel toxin BmTXK-beta	http://venomkb.org/P6822073
NTX416183
P3845530	100	0.0e+00	Potassium channel toxin alpha-KTx 14.3	http://venomkb.org/P3845530
P2286366	100	0.0e+00	Potassium channel toxin alpha-KTx 14.x	http://venomkb.org/P2286366
P7007476	91.429	0.0e+00	Potassium channel toxin alpha-KTx 14.1	http://venomkb.org/P7007476
P2547232	91.429	0.0e+00	Potassium channel toxin alpha-KTx 14.2	http://venomkb.org/P2547232
P3814049	88.571	0.0e+00	Potassium channel toxin alpha-KTx 14.5	http://venomkb.org/P3814049
NTX423614
P4952465	86.364	0.0e+00	Toxin BmKaTx16	http://venomkb.org/P4952465
P4787611	86.364	0.0e+00	Alpha-like toxin BmK-M1	http://venomkb.org/P4787611
P4052769	81.818	0.0e+00	Neurotoxin BmK-M9	http://venomkb.org/P4052769
P6523249	81.818	0.0e+00	Neurotoxin BmK-M11	http://venomkb.org/P6523249
NTX434966
P0674631	100	0.0e+00	Beta-toxin BmKAS	http://venomkb.org/P0674631
P2113503	85	0.0e+00	Beta-mammal/insect toxin Lqhb1	http://venomkb.org/P2113503
P8482100	81.667	0.0e+00	Beta-toxin BmKAs1	http://venomkb.org/P8482100
P0234296	95.122	0.0e+00	Neurotoxin MeuNaTx-6	http://venomkb.org/P0234296
NTX444153
P0774360	100	0.0e+00	Potassium channel toxin BmTXK-beta-2	http://venomkb.org/P0774360
P0326956	100	0.0e+00	Potassium channel toxin Meg-beta-KTx1	http://venomkb.org/P0326956
P5240294	86.667	0.0e+00	Potassium channel toxin MeuTXK-beta-2	http://venomkb.org/P5240294
P8357807	93.333	0.0e+00	Potassium channel toxin AaTXK-beta	http://venomkb.org/P8357807
P3133404	86.667	0.0e+00	Potassium channel toxin MeuTXK-beta-1	http://venomkb.org/P3133404
NTX512262
P9897492	97.727	0.0e+00	BmKBT-like peptide	http://venomkb.org/P9897492
P0420222	88.372	0.0e+00	Toxin BmKBT	http://venomkb.org/P0420222
NTX596227
P1446795	94.444	0.0e+00	Depressant scorpion toxin BmKIM	http://venomkb.org/P1446795
P1255792	97.222	0.0e+00	Toxin BmKAEP	http://venomkb.org/P1255792
P5127212	97.222	0.0e+00	Beta-insect depressant toxin BmKITb	http://venomkb.org/P5127212
P8255564	97.222	0.0e+00	Neurotoxin BmKAEP2	http://venomkb.org/P8255564
P2415271	94.444	0.0e+00	Beta-insect depressant toxin BmKITa	http://venomkb.org/P2415271
NTX602037
P2003384	90.909	0.0e+00	Alpha-toxin BmalphaTx47	http://venomkb.org/P2003384
P8379826	86.364	0.0e+00	Makatoxin-2	http://venomkb.org/P8379826
P8147764	90.476	0.0e+00	Makatoxin-1	http://venomkb.org/P8147764
P4052769	85.714	0.0e+00	Neurotoxin BmK-M9	http://venomkb.org/P4052769
P8173957	85.714	0.0e+00	Toxin BmKaTx13	http://venomkb.org/P8173957
NTX602038
P8379826	90.909	0.0e+00	Makatoxin-2	http://venomkb.org/P8379826
P7820336	88.636	0.0e+00	Makatoxin-3	http://venomkb.org/P7820336
P4052769	88.095	0.0e+00	Neurotoxin BmK-M9	http://venomkb.org/P4052769
P2003384	86.364	0.0e+00	Alpha-toxin BmalphaTx47	http://venomkb.org/P2003384
P8173957	88.095	0.0e+00	Toxin BmKaTx13	http://venomkb.org/P8173957
NTX627879
P0779853	100	0.0e+00	Toxin BmCa-1	http://venomkb.org/P0779853
P2897787	93.75	0.0e+00	Neurotoxin lambda-MeuTx	http://venomkb.org/P2897787
P7082344	92.188	0.0e+00	Putative calcium channel toxin Tx758	http://venomkb.org/P7082344

Download the venom annotation file

3.4 Functional annotation

Gene ontology (GO) is one the fundamental functional classification system. In this sub-section, it was applied to annotate the peptides and illustrated as a treemap below:

Download the gene ontology summary

A biological pathway is a series of interactions among molecules in a cell that leads to a certain product or a change in a cell. Such a pathway can trigger the assembly of new molecules, such as a fat or protein. Pathways can also turn genes on and off, or spur a cell to move. In the table below, all of the items with availbale pathway annotation were listed.

ID	GeneID	GeneName	Hyperlink
NTX4281	hsa:55207	ARL8B ARL10C Gie1	http://www.genome.jp/dbget-bin/www_bget?hsa:55207
NTX13418	hsa:2195	FAT1 CDHF7 CDHR8 FAT ME5 hFat1	http://www.genome.jp/dbget-bin/www_bget?hsa:2195
NTX24145	hsa:70	ACTC1 ACTC ASD5 CMD1R CMH11 LVNC4	http://www.genome.jp/dbget-bin/www_bget?hsa:70
NTX38478	hsa:71	ACTG1 ACT ACTG BRWS2 DFNA20 DFNA26 HEL-176	http://www.genome.jp/dbget-bin/www_bget?hsa:71
NTX56407	hsa:4925	NUCB2 HEL-S-109 NEFA	http://www.genome.jp/dbget-bin/www_bget?hsa:4925
NTX94322	hsa:3309	HSPA5 BIP GRP78 HEL-S-89n MIF2	http://www.genome.jp/dbget-bin/www_bget?hsa:3309
NTX102642	hsa:71	ACTG1 ACT ACTG BRWS2 DFNA20 DFNA26 HEL-176	http://www.genome.jp/dbget-bin/www_bget?hsa:71
NTX132748	hsa:554313	HIST2H4B H4/o	http://www.genome.jp/dbget-bin/www_bget?hsa:554313
NTX132749	hsa:554313	HIST2H4B H4/o	http://www.genome.jp/dbget-bin/www_bget?hsa:554313
NTX136251	hsa:102	ADAM10 AD10 AD18 CD156c CDw156 HsT18717 MADM RAK kuz	http://www.genome.jp/dbget-bin/www_bget?hsa:102
NTX142744	hsa:5648	MASP1 3MC1 CRARF CRARF1 MAP1 MASP MASP3 MAp44 PRSS5 RaRF	http://www.genome.jp/dbget-bin/www_bget?hsa:5648
NTX148639	hsa:60	ACTB BRWS1 PS1TP5BP1	http://www.genome.jp/dbget-bin/www_bget?hsa:60
NTX173892	hsa:71	ACTG1 ACT ACTG BRWS2 DFNA20 DFNA26 HEL-176	http://www.genome.jp/dbget-bin/www_bget?hsa:71
NTX177486	hsa:11093	ADAMTS13 ADAM-TS13 ADAMTS-13 C9orf8 VWFCP vWF-CP	http://www.genome.jp/dbget-bin/www_bget?hsa:11093
NTX178649	hsa:90411	MCFD2 F5F8D F5F8D2 LMAN1IP SDNSF	http://www.genome.jp/dbget-bin/www_bget?hsa:90411
NTX186740	hsa:7170	TPM3 CAPM1 CFTD HEL-189 HEL-S-82p NEM1 OK/SW-cl.5 TM-5 TM3 TM30 TM30nm TM5 TPMsk3 TRK hscp30	http://www.genome.jp/dbget-bin/www_bget?hsa:7170
NTX189949	hsa:726	CAPN5 ADNIV HTRA3 VRNI nCL-3	http://www.genome.jp/dbget-bin/www_bget?hsa:726
NTX212167	hsa:51121	RPL26L1 RPL26P1	http://www.genome.jp/dbget-bin/www_bget?hsa:51121
NTX213988	hsa:653604	HIST2H3D	http://www.genome.jp/dbget-bin/www_bget?hsa:653604
NTX214579	hsa:347733	TUBB2B PMGYSA bA506K6.1	http://www.genome.jp/dbget-bin/www_bget?hsa:347733
NTX226741	hsa:8340	HIST1H2BL H2B/c H2BFC dJ97D16.4	http://www.genome.jp/dbget-bin/www_bget?hsa:8340
NTX227449	hsa:6189	RPS3A FTE1 MFTL S3A	http://www.genome.jp/dbget-bin/www_bget?hsa:6189
NTX235909	hsa:5034	P4HB CLCRP1 DSI ERBA2L GIT P4Hbeta PDI PDIA1 PHDB PO4DB PO4HB PROHB	http://www.genome.jp/dbget-bin/www_bget?hsa:5034
NTX235912	hsa:5034	P4HB CLCRP1 DSI ERBA2L GIT P4Hbeta PDI PDIA1 PHDB PO4DB PO4HB PROHB	http://www.genome.jp/dbget-bin/www_bget?hsa:5034
NTX248037	hsa:805	CALM2 CAMII LQT15 PHKD PHKD2 caM	http://www.genome.jp/dbget-bin/www_bget?hsa:805
NTX258811	hsa:60	ACTB BRWS1 PS1TP5BP1	http://www.genome.jp/dbget-bin/www_bget?hsa:60
NTX260492	hsa:1506	CTRL CTRL1	http://www.genome.jp/dbget-bin/www_bget?hsa:1506
NTX270040	hsa:2597	GAPDH G3PD GAPD HEL-S-162eP	http://www.genome.jp/dbget-bin/www_bget?hsa:2597
NTX283712	hsa:3854	KRT6B CK-6B CK6B K6B KRTL1 PC2 PC4	http://www.genome.jp/dbget-bin/www_bget?hsa:3854
NTX317140	hsa:5651	TMPRSS15 ENTK PRSS7	http://www.genome.jp/dbget-bin/www_bget?hsa:5651
NTX324164	hsa:10383	TUBB4B Beta2 TUBB2 TUBB2C	http://www.genome.jp/dbget-bin/www_bget?hsa:10383
NTX334429	hsa:5986	RFNG	http://www.genome.jp/dbget-bin/www_bget?hsa:5986
NTX335822	hsa:3998	LMAN1 ERGIC-53 ERGIC53 F5F8D FMFD1 MCFD1 MR60 gp58	http://www.genome.jp/dbget-bin/www_bget?hsa:3998
NTX336196	hsa:8345	HIST1H2BH H2B/j H2BFJ	http://www.genome.jp/dbget-bin/www_bget?hsa:8345
NTX352134	hsa:8708	B3GALT1 beta3Gal-T1	http://www.genome.jp/dbget-bin/www_bget?hsa:8708
NTX361043	hsa:5651	TMPRSS15 ENTK PRSS7	http://www.genome.jp/dbget-bin/www_bget?hsa:5651
NTX430388	hsa:5110	PCMT1 PIMT	http://www.genome.jp/dbget-bin/www_bget?hsa:5110
NTX431293	hsa:7178	TPT1 HRF TCTP p02 p23	http://www.genome.jp/dbget-bin/www_bget?hsa:7178
NTX438454	hsa:140606	SELM SEPM	http://www.genome.jp/dbget-bin/www_bget?hsa:140606
NTX465514	hsa:3856	KRT8 CARD2 CK-8 CK8 CYK8 K2C8 K8 KO	http://www.genome.jp/dbget-bin/www_bget?hsa:3856
NTX468692	hsa:10975	UQCR11 0710008D09Rik QCR10 UQCR	http://www.genome.jp/dbget-bin/www_bget?hsa:10975
NTX487637	hsa:5122	PCSK1 BMIQ12 NEC1 PC1 PC3 SPC3	http://www.genome.jp/dbget-bin/www_bget?hsa:5122
NTX487647	hsa:5122	PCSK1 BMIQ12 NEC1 PC1 PC3 SPC3	http://www.genome.jp/dbget-bin/www_bget?hsa:5122
NTX494486	hsa:170692	ADAMTS18 ADAMTS21 KNO2 MMCAT	http://www.genome.jp/dbget-bin/www_bget?hsa:170692
NTX519666	hsa:6208	RPS14 EMTB S14	http://www.genome.jp/dbget-bin/www_bget?hsa:6208
NTX523065	hsa:5126	PCSK2 NEC_2 NEC-2 NEC2 PC2 SPC2	http://www.genome.jp/dbget-bin/www_bget?hsa:5126
NTX541125	hsa:59084	ENPP5 NPP-5	http://www.genome.jp/dbget-bin/www_bget?hsa:59084
NTX546099	hsa:5045	FURIN FUR PACE PCSK3 SPC1	http://www.genome.jp/dbget-bin/www_bget?hsa:5045
NTX558144	hsa:4697	NDUFA4 CI-9k CI-MLRQ MLRQ	http://www.genome.jp/dbget-bin/www_bget?hsa:4697
NTX577282	hsa:3856	KRT8 CARD2 CK-8 CK8 CYK8 K2C8 K8 KO	http://www.genome.jp/dbget-bin/www_bget?hsa:3856
NTX584753	hsa:5651	TMPRSS15 ENTK PRSS7	http://www.genome.jp/dbget-bin/www_bget?hsa:5651
NTX629813	hsa:23071	ERP44 PDIA10 TXNDC4	http://www.genome.jp/dbget-bin/www_bget?hsa:23071
NTX644816	hsa:50487	PLA2G3 GIII-SPLA2 SPLA2III sPLA2-III	http://www.genome.jp/dbget-bin/www_bget?hsa:50487
NTX644817	hsa:50487	PLA2G3 GIII-SPLA2 SPLA2III sPLA2-III	http://www.genome.jp/dbget-bin/www_bget?hsa:50487

Download the pathway summary

3.4 Similarity search

A BLAST search allows users to infer the function of a sequence from similar sequences. A tab-delimited table is available from the link below.

Download the tabular table of BLAST

Besides, this tool also provides a graphic summary and some description for each alignment between the query sequence and the sequence hits from the database.

Click me to view the detail

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